Composite

Part:BBa_K4324300:Design

Designed by: Chris Yoo   Group: iGEM22_TheKingsSchool_AU_HS   (2022-09-30)


XR-XDH-XK-PK for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1649
    Illegal BglII site found at 4065
    Illegal BamHI site found at 4362
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5767
    Illegal AgeI site found at 2929
    Illegal AgeI site found at 3217
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1306
    Illegal SapI.rc site found at 747


Design Notes

The protein sequence was codon-optimised for Escherichia coli through the [IDT codon optimisation tool https://sg.idtdna.com/CodonOpt] as gBlocks Gene Fragments.

TBA


Source

Uniprot TBA

References